 |
|
 |
After reaching the correct pH, I added the agar (gelling agent) at 7 g/L, then placed the media in the autoclave. Sterilizing all tools, media, and beakers are essential for successful micropropagation. This step takes about 25 minutes for a liquid cycle. The machine heats and pressurizes its contents at 250F degrees and 15 pounds per square inch, killing any bacteria or fungus will disrupt the growth of explants. |
 |
|
These are some of the dials seen on our autoclave here at TREC.
|